J Paediatr Child Health. 2021 Dec 6. doi: 10.1111/jpc.15845. Online ahead of print.

Madison Hill ¹, Runa Watkins ¹, Elaine Leonard-Puppa ¹, Jaylyn Waddell ², Samra Blanchard ¹, Howard Kader ¹

Author information

¹Department of Pediatrics, Division of Gastroenterology and Nutrition, University of Maryland Medical Center, Baltimore, Maryland, United States.

²Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, United States.

Abstract

Aim: The standard serological test to screen for coeliac disease (CD) is tissue transglutaminase (tTG) but some experts recommend including deamidated gliadin peptide (DGP) antibodies for children younger than 3 years old. This study evaluated the utility of DGP-immunoglobulin A (IgA) and DGP-immunoglobulin G (IgG) serologies when screening children younger than 3 years old for CD.

Methods: A retrospective chart review was conducted including children 3 years old and under, who had DGP and/or tTG serologies along with duodenal biopsies during their initial diagnostic evaluation. Serology results were compared to the gold-standard histopathology by χ² to determine the significance of including DGP-IgG/IgA serologies when screening for CD in this age group.

Results: We identified 478 patients, 52 who were younger than 3 years old, 43 of whom met inclusion criteria. The positive predictive value (PPV) of the DGP-IgA test was 91.7% whereas, DGP-IgG was 77.8%. When DGP serology was examined in conjunction with tTG-IgA, the PPV with DGP-IgA was 90.9% and with DGP-IgG was 87.5%.

Conclusions: In isolation, DGP-IgA provides a high PPV and specificity for CD in children younger than 3 years old, whereas DGP-IgG had a much lower PPV in this age group. When used alone or in conjunction with tTG-IgA, the DGP-IgA test results in a high PPV of 91.7 and 90.9%, respectively. Based on our study, we recommend obtaining both the DGP-IgA and the tTG-IgA serology when screening infants and children younger than 3 years old for coeliac disease.